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Target region sequencing is a cost effective and powerful method for researches on specific regions of a genome or genes. It contains probes designing, target region capture, enrichment, pre-end sequencing and Bioinformatics analysis based on target sequences. By enrichment of target regions fragments, a deep and high-coverage sequencing can be performed with low sequencing amount. Which makes it suitable for larger sample size sample analysis, disease or the whole genome and GWAS analysis.