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Q: Single cell genome sequencing sample requirements What’re the requirements of single cell genome sequencing sample?

A: MDA amplification: the sample volume should not  be more than 2μl, tubes containing 2μl PBS are provided. MALBAC amplification: the sample volume should not be more than 1μl, ensure there is no Ca2+, Mg2+ together with the sample. 4μl lysis solution is provided. Please make the single cell genome samples in a separate state one by one. Avoiding the cell adhesion and cell debris, which may affect the quality of amplification. The stored cells should be shipped out with dry ice.

     

Q: How to assess the single cell genome sequencing coverage?

A: When single-cell whole genome scWGSsample library construction finished. First, we need sequencing a small amount of data (example: human, data 2G), and then assess the test data with software (Preseq) for 30X, you should according to the evaluation results determine whether to add the sequencing or re-send the sample.

     A small amount of data will be sequenced  right after single-cell whole genome scWGSsample library construction finished, then the test data will be assessed with software (Preseq) for 30X. According to the results of the assessment, the customer could decide whether to carry out the sequencing or re-send the samples.