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Q: What are the advantages of the transcriptome sequencing?

A: 1) High accuracy, enable direct read of each transcript at the single nucleotide resolution;

     2) High sensitivity, have the capability to detect the rare-copy transcript;

     3)  Without species limitations;

     4) Wide range of detection:  high dynamic with six levels, ranging from rare-copy transcript to high-copy  transcript.

Q:  Compared with microarray, what’s the advantage of transcriptome sequencing ?

A: 1) Microarray analysis can only get the known information because of the probe capturing principle, while the transcriptome sequencing have the ability to read all the transcript information including the known and the novel transcript;

     2) RNA-seq have a higher detection rate of differentially expressed genes than that of   microarray (93% VS 75%);

     3) RNA-seq is more sensitive towards the lower abundance of  differentially expressed genes than microarray (89% VS 17%);

    4) The correlation of expression result between RNA-seq and qPCR is much higher than that between microarray and qPCR.