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Q:Sequencer parameter


Table 5. Parameter of HiSeq X Ten

Table6. Parameter of NovaSeq 6000 S4

Q:Processes of library qualifying

A:Agilent 2100 —— StepOnePlus™ Real-Time PCR System

Q:Matters need attention

A:1) The structure of libraries should meet the requirements of Illumina sequencers.

       2) Please ship samples as follows:

       a. Samples need to be packed in 1.5 mL centrifuge tubes with parafilm to prevent loss.

       b. Volume of library should be more than 10 μL.