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Long non-coding RNA (LncRNA) are transcribed RNAs longer than 200nt that do not encode for proteins, which is widely distributed in organisms. It is able to acquire the LncRNA sequence information with single-base resolution for one time via the high-throughput sequencing by constructing the strand-specific library for the removal of rRNA, and identify the known LncRNA and predict the new one and its target region depended on the powerful bioinformatics analysis platform.

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