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Whole Genome ExomeSequencing (WES) is used to probe out the genetic mutation related to the functional variation of proteins by high-throughput sequencing with the probe hybridization for the DNA sequences in the protein coding region. WES is available at a lower cost than WGS, with more advantages for research on the SNP and InDel of the known genes. It is applied to the studies of cancer, genetic diseases and complex diseases, etc. It is likely to find common and rare mutations through deep sequencing, which provides important technical support for the studies relating to disease mechanism and gene function.